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A family of contact-dependent nuclease effectors contain an exchangeable, species-identifying domainÂ
A family of contact-dependent nuclease effectors contain an exchangeable, species-identifying domainÂ
Denise Sirias1, Daniel R. Utter2, and Karine A. Gibbs1,*.
1Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge MA 02138, USA.
2Department of Organismic and Evolutionary Biology, Harvard University, 16 Divinity Avenue, Cambridge MA 02138, USA.
*For correspondence: kagibbs [at] mcb [.] harvard [.] edu
NB: The manuscript is an unpublished draft, also available on bioRxiv as a preprint (doi: https://doi.org/10.1101/2020.02.20.956912): https://biorxiv.org/cgi/content/short/2020.02.20.956912v1.
Computational methods are provided at <Sirias et al. 2020 - narrative methods>
Sirias et al_bioRxiv.pdfGrowth recovery assay and FACS-based population sorting following territorial exclusion in Proteus mirabilis
Growth recovery assay and FACS-based population sorting following territorial exclusion in Proteus mirabilis
Murray J. Tipping* and Karine A. Gibbs
Department of Molecular and Cellular Biology, Harvard University, Cambridge MA, USA
*For correspondence: mjt [at] tippings [.] net
NB: The manuscript is an unpublished draft.
Tipping-and-Gibbs_FACSprotocol-VidFig1.mp4TippingAndGibbs_FACSprotocol_unpublished-draft.pdfThe FACS protocol is based on the research published in Tipping and Gibbs (2019) "Peer pressure from a Proteus mirabilis self-recognition system controls participation in cooperative swarm motility" <https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1007885>.
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