Gibbs Lab

Deciphering self versus non-self recognition in bacteria

Cells from two different populations of Proteus mirabilis meeting as they migrate as a swarm along the rigid surface.

In the Gibbs research group, our goal is to identify the mechanisms that underlie self versus non-self recognition in cells. Self recognition is an essential building block of nature observed at many levels of biological complexity ranging from immune cells to bacteria. Migrating colonies of independent Proteus mirabilis isolates recognize each other as foreign and do not merge together when they intersect, whereas colonies of clonal isolates do merge, indicating that this bacterium is capable of self/non-self recognition (see movie). P. mirabilis is broadly found in animal and human intestines and can cause bladder and kidney infections.

We strive to harness this bacterium’s relative genetic simplicity and tractability to discern the molecular mechanisms underlying its self/non-self recognition process. We leverage its sophisticated synthesis of sensing, signaling, and motility to advance understanding of the basic phenomena that give rise to social behaviors, such as cell-cell communication, cooperative migration, competition, and pathogenesis. Our research broadly addresses how cells define themselves, communicate their self-identity, and respond to others.

Colonies of P. mirabilis migrate as swarms on top of a rigid nutrient surface. The pattern of concentric circles is characteristic of a P. mirabilis swarm.

Left: Each colony was isolated from a different person. A visible line forms between the two colonies and is apparent at the end of the time-lapse movie.

Right: Each colony was isolated from the same person. The two colonies form a single, coherent colony at the end of the time-lapse movie.

Movies taken by J. Austerman.

Interested in joining our group for a postdoctoral fellowship or research internship? Contact Dr. Gibbs directly at kagibbs [at] mcb {dot} harvard {dot} edu.

To attend graduate school, apply through the Molecules, Cells, and Organisms graduate program (MCO) at Harvard University.

A list of recent publications can be found at https://orcid.org/0000-0002-1246-6401.